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KMID : 0379119900180030115
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Korean Journal of Mycology
1990 Volume.18 No. 3 p.115 ~ p.126
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Studies on Protoplast Formation and Regeneration of Coriolus versicolor
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Abstract
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To establish basic techniques for protoplast fusion of Coriolua versicolor several factors affecting protoplast formation and regeneration were investigated. Protoplast isolation was at maximum with 2.5-day cultured mycelia of C. versicolor treated with the combination of two enzymes, Novozym 234 (10 ㎎/㎖) and cellulase Onozuka R-10 (15 ㎎/㎖), for 3-4.5 hours at 30℃. As an osmotic stabilizer for stabilizing the protoplast, 0.6 M sucrose was the best for formation and regeneration of the protoplast from the mycelia ofthe fungus and the regeneration frequency was 3.48%. Protoplast fusion was made by a modified method of Peberdy using PEG (M.W. 4,000). The fusion frequency between two mutants of C. versicolor was 1.86% and the fusion products showed differences in growth rate and colony morphology.
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